| Num. Piloti | Team |
| 46 |  | Valentino Rossi
Campione del Mondo in carica | | 99 |  | Jorge Lorenzo |
| Fiat Yamaha - Yamaha |
| 3 |  |
Dani Pedrosa
| | 4 |  | Andrea Dovizioso |
| Honda Repsol - Honda |
| 27 |  | Casey Stoner | | 69 |  | Nicky Hayden |
| Ducati Marlboro Team - Ducati |
| 33 |  | Marco Melandri |
| Hayate Racing Team
Kawasaki |
| 15 |  | Alex de Angelis | | 24 |  | Toni Elias |
| Honda Gresini - Honda |
| 5 |  | Colin Edwards | | 52 |  | James Toseland |
| Yamaha Tech3 – Yamaha |
| 7 |  | Chris Vermeulen | | 65 |  | Loris Capirossi |
| Rizla Suzuki – Suzuki |
| 36 |  | Mika Kallio | | 88 |  | Niccolō Canepa |
| Pramac Racing
Ducati |
| 59 |  | Sete Gibernau |
| Guinea Ecuatorial
Team - Ducati |
| 72 |  | Yuki Takahashi | | 41 |  | Gabor Talmacsi
|
| JiR Scot Team – Honda |
| 14 |  | Randy de Puniet |
| Honda LCR – Honda
|
